Journal Articles

Ratcliffe, E, Hourd, P, Guijarro-Leach, J, Rayment, E, Williams, DJ, Thomas, RJ (2013) Application of response surface methodology to maximize the productivity of scalable automated human embryonic stem cell manufacture., Regen Med, 8(1), pp.39-48, DOI: 10.2217/rme.12.109.

Ratcliffe, E, Glen, KE, Workman, VL, Stacey, AJ, Thomas, RJ (2012) A novel automated bioreactor for scalable process optimisation of haematopoietic stem cell culture., J Biotechnol, 161(3), pp.387-390, DOI: 10.1016/j.jbiotec.2012.06.025.

Gould, T, Saeed, AO, Thomas, R, Rose, F, Shakesheff, K (2012) Technologies for live stem cell transportation, JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 6, pp.412-412, ISSN: 1932-6254.

Williams, DJ, Thomas, RJ, Hourd, PC, Chandra, A, Ratcliffe, E, Liu, Y, Rayment, EA, Archer, JR (2012) Precision manufacturing for clinical-quality regenerative medicines., Philos Trans A Math Phys Eng Sci, 370(1973), pp.3924-3949, ISSN: 1364-503X. DOI: 10.1098/rsta.2011.0049.

Thomas, R and Ratcliffe, E (2012) Automated adherent human cell culture (mesenchymal stem cells), Methods in Molecular Biology, 806, pp.393-406, ISSN: 1064-3745. DOI: 10.1007/978-1-61779-367-7_26.

Gould, TWA, Rose, FRAJ, Shakesheff, KM, Thomas, RJ (2011) Cell life support system for transportation, European Cells and Materials, 22(SUPPL.3), pp.62-62, ISSN: 1473-2262.

Ratcliffe, E, Thomas, RJ, Williams, DJ (2011) Current understanding and challenges in bioprocessing of stem cell-based therapies for regenerative medicine., Br Med Bull, 100, pp.137-155, DOI: 10.1093/bmb/ldr037.

Hewitt, CJ, Lee, K, Nienow, A, Thomas, RJ, Smith, M, Thomas, CR (2011) Expansion of human mesenchymal stem cells on microcarriers, Biotechnology Letters, 33, pp.2325-3335, DOI: 10.1007/s10529-011-0695-4.

Thomas, RJ and Williams, DJ (2009) Large-Scale Manufacture of Therapeutic Human Stem Cells, Pharmaceutical Technology, 33(7), pp.74-79, Full text: http://pharmtech.findpharma.com/pharmtech/Article/Large-Scale-Manufacture-of-Therapeutic-Human-Stem-/ArticleStandard/Article/detail/608705 .

Thomas, RJ, Anderson, D, Chandra, A, Smith, NM, Young, LE, Williams, DJ, Denning, C (2009) Automated, Scalable Culture of Human Embryonic Stem Cells in Feeder-Free Conditions, Biotechnology and Bioengineering, 102(6), pp.1636-1644, Full text: http://dx.doi.org/10.1002/bit.22187. DOI: 10.1002/bit.22187.

Thomas, RJ, Hope, AD, Hourd, P, Baradez, M, Miljan, EA, Sinden, JD, Williams, DJ (2009) Automated, serum-free production of CTX0E03: a therapeutic clinical grade human neural stem cell line, Biotechnology Letters, Online First, pp.1-6, ISSN: 1573-6776. Full text: http://dx.doi.org/10.1007/s10529-009-9989-1. DOI: 10.1007/s10529-009-9989-1.

Thomas, R (2009) Regenerative medicine: where are we at?, JOURNAL OF PHARMACY AND PHARMACOLOGY, 61, pp.A165-A165, ISSN: 0022-3573.

Thomas, RJ, Hourd, P, Williams, DJ (2008) Application of process quality engineering techniques to improve the understanding of the in-vitro processing of stem cells for therapeutic use, Journal of Biotechnology, 136(3-4), pp.148-155, Full text: http://dx.doi.org/10.1016/j.jbiotec.2008.06.009. DOI: 10.1016/j.jbiotec.2008.06.009.

Thomas, RJ, Chandra, A, Hourd, P, Williams, DJ (2008) Cell Culture Automation and Quality Engineering: A Necessary Partnership to Develop Optimised Manufacturing Processes for Cell-Based Therapies, Journal of the Association of Laboratory Automation, 13(3), pp.152-158, Full text: http://dx.doi.org/10.1016/j.jala.2007.12.003. DOI: 10.1016/j.jala.2007.12.003.

Mather, ML, Crowe, JA, Morgan, SP, White, LJ, Shakesheff, KM, Howdle, SM, Thomas, RJ, Williams, DJ, Byrne, HM, Waters, SL (2008) Remedi: A research consortium applying engineering strategies to establish regenerative medicine as a new industry, IFMBE Proceedings, 22, pp.2209-2212, ISSN: 1680-0737. DOI: 10.1007/978-3-540-89208-3_528.

Thomas, RJ, Chandra, A, Liu, Y, Hourd, P, Conway, PP, Williams, DJ (2007) Manufacture of a human mesenchymal stem cell population using an automated cell culture platform, Cytotechnology, 55(1), pp.31-39, ISSN: 0920-9069. Full text: http://dx.doi.org/10.1007/s10616-007-9091-2. DOI: 10.1007/s10616-007-9091-2.

Thomas, RJ, Bennett, A, Thomson, B, Shakesheff, K (2006) Hapatic stellate cells on poly(DL-lactic acid) surfaces control the formation of 3D hepatocyte co-culture aggregates in vitro, European Cells and Materials, 11, pp.16-26.

Thomas, RJ, Bennett, A, Thomson, B, Shakesheff, KM (2006) Hepatic stellate cells on poly(DL-lactic acid) surfaces control the formation of 3D hepatocyte co-culture aggregates in vitro., Eur Cell Mater, 11, pp.16-26.

Thomas, RJ, Bhandari, R, Barrett, DA, Bennett, AJ, Fry, JR, Powe, D, Thomson, BJ, Shakesheff, KM (2005) The effect of three-dimensional co-culture of hepatocytes and hepatic stellate cells on key hepatocyte functions in vitro, Cells, Tissues, Organs, 181(2), pp.67-79.

Thomas, RJ, Bhandari, R, Barrett, DA, Bennett, AJ, Fry, JR, Powe, D, Thomson, BJ, Shakesheff, KM (2005) The effect of three-dimensional co-culture of hepatocytes and hepatic stellate cells on key hepatocyte functions in vitro., Cells Tissues Organs, 181(2), pp.67-79, ISSN: 1422-6405. DOI: 10.1159/000091096.

Thomas, R, Woon, ML, Ralevic, V, Alexander, SPH (2002) An investigation of the role of cyclic nucleotides and potassium channels in the ADP-induced relaxation of the porcine isolated coronary artery, BRITISH JOURNAL OF PHARMACOLOGY, 135, ISSN: 0007-1188.

Conference Proceedings

Williams, DJ, Guijarro-Leach, JJ, Ratcliffe, E, Denning, C, Thomas, RJ, Young, L (2011) Investigating the Consistent Scaled Processing of Human Embryonic Stem Cells (2.P6). In TERMIS-EU 2011, Histology and Histopathology: cellular and molecular biology, Granada, Spain, pp.29-29.

Williams, DJ, Iftimia, AD, Irving, J, Thomas, RJ (2011) Optimization of Processing and Expansion for Mesenchymal Stem Cells from Umbilical Cord Tissue (48.P5). In TERMIS-EU 2011, Histology and Histopathology: cellular and molecular biology, Granada, Spain, pp.397-397.

Mather, ML, Crowe, JA, Thomas, RJ, Waters, SL, Byrne, HM (2008) Mathematical Investigation of Population Variability in Neural Stem Cells. In Meeting of the Tissue and Cell Engineering Society (TCES) 2-4 July 2008, European Cells & Materials Journal, University of Nottingham, UK, p.81, Full text: http://www.ecmjournal.org/journal/supplements/vol016supp03/pdf/v016supp03a081.pdf .

Singh, P, Thomas, RJ, Williams, DJ (2008) (OP 21) Automated Culture of Umbilical Cord Blood Derived Progenitor Cells. In Termis EU 2008, Tissue Engineering: Part A: Meeting Abstracts, Porto, Portugal, p.701, Full text: http://dx.doi.org/10.1089/tea.2008.1504. DOI: 10.1089/tea.2008.1504.

Thomas, RJ, Chandra, A, Hourd, P, Williams, DJ (2008) (OP 22) Automation, Scale-up and Process Engineering: Essentials for Successful Translation of Cell Based Therapies. In Termis EU 2008, Tissue Engineering: Part A: Meeting Abstracts, Porto, Portugal, pp.701-702, Full text: http://dx.doi.org/10.1089/tea.2008.1504. DOI: 10.1089/tea.2008.1504.

Singh, P, Thomas, RJ, Williams, DJ (2008) Automated culture of umbilical cord blood derived progenitor cells. In Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, TISSUE ENGINEERING PART A, Porto, PORTUGAL, pp.701-701.

Thomas, RJ, Chandra, A, Hourd, PC, Williams, DJ (2008) Automation, scale-up and process engineering: Essentials for successful translation of cell based therapies. In Annual Tissue-Engineering-and-Regenerative-Medicine-International-Society-European-Chapter Meeting, TISSUE ENGINEERING PART A, Porto, PORTUGAL, pp.701-702.

Thomas, RJ, Chandra, A, Williams, DJ (2007) Culture of Human Mesenchymal Stem Cells Using a Novel Automated Cell Culture Platform. In Termis NA 2007, Proceedings of Termis NA 2007, Toronto, Canada, pp.31-39.

Hammond, JS, Thomas, R, Barry, J, Beckingham, IJ, Shakesheff, KM (2005) Synthesis of functional liver tissue using a novel tissue engineering strategy.. In Termis NA 2007, HEPATOLOGY, pp.506A-507A.

Department Working Papers

Hourd, P and Thomas, RJ (2007) hMSC DOE Experimental Details.

Hourd, P and Thomas, RJ (2007) hMSC Process DOE Design.

Thomas, RJ and Hourd, P (2007) Process Characterisation of the Automated Cell Culture of MSCs (CompacT SelecT): Process Map.

Liu, Y, Hourd, P, Thomas, RJ, Shakesheff, K, Archer, R, Williams, DJ (2006) Challenges of Culturing Functioning Cells in-vitro for Regenerative Medicine and Stem Cell Therapy from a Process Perspective.

Thomas, RJ, Liu, Y, Chandra, A, Hourd, P, Williams, DJ (2006) Automated hMSC Culture Using the Compact SelecT.