Journal Articles
Bahsoun, S, Brown, M-J,
Coopman, K, Akam, EC (2022)
Cryopreservation of human bone marrow derived mesenchymal stem cells at high concentration is feasible,
Biopreservation and Biobanking, 21(5), pp.450-457, ISSN: 1947-5535. DOI:
10.1089/bio.2022.0017.
Grant, R,
Coopman, K, Silva-Gomes, S, Campbell, J, Kara, B, Braybrook, J, Petzing, J (2021)
Assessment of protocol impact on subjectivity uncertainty when analyzing peripheral blood mononuclear cell flow cytometry data files,
Methods and Protocols, 4(2), 24, ISSN: 2409-9279. DOI:
10.3390/mps4020024.
Englezakis, A, Gozalpour, E, Kamran, M, Fenner, K, Mele, E,
Coopman, K (2021)
Development of a hollow fibre-based renal module for active transport studies,
Journal of Artificial Organs, 24, pp.473-484, ISSN: 1434-7229. DOI:
10.1007/s10047-021-01260-w.
Grant, R,
Coopman, K, Medcalf, N, Silva-Gomes, S, Campbell, JJ, Kara, B, Braybrook, J, Petzing, J (2021)
Quantifying operator subjectivity within flow cytometry data analysis as a source of measurement uncertainty and the impact of experience on results,
PDA Journal of Pharmaceutical Science and Technology, 75(1), pp.33-47, ISSN: 1079-7440. DOI:
10.5731/pdajpst.2019.011213.
Bahsoun, S,
Coopman, K, Akam, L (2020)
Quantitative assessment of the impact of cryopreservation on human bone marrow-derived mesenchymal stem cells: up to 24 h post-thaw and beyond,
Stem Cell Research and Therapy, 11, 540, ISSN: 1757-6512. DOI:
10.1186/s13287-020-02054-2.
Hanga, M, Nienow, A, Murasiewicz, H, Pacek, A, Hewitt, C,
Coopman, K (2020)
Expansion of human mesenchymal stem/stromal cells on temporary liquid microcarriers,
Journal of Chemical Technology and Biotechnology, 96(4), pp.930-940, ISSN: 0268-2575. DOI:
10.1002/jctb.6601.
Bahsoun, S,
Coopman, K, Akam, L (2019)
The impact of cryopreservation on bone marrow-derived mesenchymal stem cells: a systematic review,
Journal of Translational Medicine, 17(1), 397, DOI:
10.1186/s12967-019-02136-7.
Grant, B,
Coopman, K, Medcalf, N, Silva-Gomes, S, Campbell, J, Kara, B, Braybrook, J, Petzing, J (2019)
Understanding the contribution of operator measurement variability within flow cytometry data analysis for quality control of cell and gene therapy manufacturing,
Measurement, 150, 106998, ISSN: 0263-2241. DOI:
10.1016/j.measurement.2019.106998.
Heathman, TRJ, Nienow, AW, Rafiq, QA,
Coopman, K, Kara, B, Hewitt, CJ (2018)
Development of a process control strategy for the serum-free microcarrier expansion of human mesenchymal stem cells towards cost-effective and commercially viable manufacturing,
Biochemical Engineering Journal, 141, pp.200-209, ISSN: 1369-703X. DOI:
10.1016/j.bej.2018.10.018.
Bahsoun, S,
Coopman, K, Forsyth, NR, Akam, EC (2018)
The Role of Dissolved Oxygen Levels on Human Mesenchymal Stem Cell Culture Success, Regulatory Compliance, and Therapeutic Potential,
Stem Cells and Development, 27(19), pp.1303-1321, ISSN: 1547-3287. DOI:
10.1089/scd.2017.0291.
Heathman, TRJ, Nienow, AW, Rafiq, QA,
Coopman, K, Kara, B, Hewitt, CJ (2018)
Agitation and aeration of stirred-bioreactors for the microcarrier culture of human mesenchymal stem cells and potential implications for large-scale bioprocess development,
Biochemical Engineering Journal, 136, pp.9-17, ISSN: 1369-703X. DOI:
10.1016/j.bej.2018.04.011.
Rafiq, QA, Ruck, S, Hanga, MP, Heathman, TRJ,
Coopman, K, Nienow, AW, Williams, D, Hewitt, CJ (2017)
Qualitative and quantitative demonstration of bead-to-bead transfer with bone marrow-derived human mesenchymal stem cells on microcarriers: utilising the phenomenon to improve culture performance,
Biochemical Engineering Journal, 135, pp.11-21, ISSN: 1369-703X. DOI:
10.1016/j.bej.2017.11.005.
Rafiq, QA, Hanga, MP, Heathman, TRJ,
Coopman, K, Nienow, AW, Williams, D, Hewitt, CJ (2017)
Process development of human multipotent stromal cell microcarrier culture using an automated high-throughput microbioreactor,
Biotechnology and Bioengineering, 114(10), DOI:
10.1002/bit.26359.
Rafiq, QA, Nienow, AW, Hewitt, CJ,
Coopman, K (2017)
The role of biopreservation in cell and gene therapy bioprocessing,
Cell & Gene Therapy Insights, DOI:
10.18609/cgti.2017.037.
Odeleye, AOO, Castillo-Avila, S, Boon, M, Martin, H,
Coopman, K (2017)
Development of an optical system for the non-invasive tracking of stem cell growth on microcarriers,
Biotechnology and Bioengineering, ISSN: 0006-3592. DOI:
10.1002/bit.26328.
Hanga, MP, Murasiewicz, H, Pacek, AW, Nienow, AW,
Coopman, K, Hewitt, CJ (2017)
Expansion of bone marrow derived human mesenchymal stem/stromal cells (hMSC) using a two phase liquid/liquid system,
Journal of Chemical Technology & Biotechnology, ISSN: 0268-2575. DOI:
10.1002/jctb.5279.
Stacey, G, Connon, CJ,
Coopman, K, Dickson, AJ, Fuller, B, Hunt, CJ, Kemp, P, Kerby, J, Man, J, Matejtschuk, P, Moore, H, Morris, J, Oreffo, ROC, Slater, NKH, Ward, S, Wiggins, C, Zimmermann, H (2017)
Preservation and stability of cell therapy products: recommendations from an expert workshop,
Regenerative Medicine, ISSN: 1746-0751. DOI:
10.2217/rme-2017-0073.
Moutsatsou, P,
Coopman, K, Georgiadou, S (2017)
Biocompatibility assessment of conducting PANI/Chitosan nanofibers for wound healing applications,
Polymers, 9(12), ISSN: 2073-4360. DOI:
10.3390/polym9120687.
Rafiq, Q,
Coopman, K, Nienow, A, Hewitt, CJ (2016)
Systematic microcarrier screening and agitated culture conditions improves human mesenchymal stem cell yield in bioreactors,
Biotechnology Journal, 11(4), pp.473-486, ISSN: 1860-7314. DOI:
10.1002/biot.201400862.
Nienow, AW, Hewitt, CJ, Heathman, T, Glyn, VAM, Fonte, G, Hanga, P,
Coopman, K, Rafiq, QA (2016)
Agitation conditions for the culture and detachment of hMSCs from microcarriers in multiple bioreactor platforms,
Biochemical Engineering Journal, ISSN: 1873-295X.
Heathman, T, Stolzing, A, Fabian, C, Rafiq, QA,
Coopman, K, Nienow, AW, Kara, B, Hewitt, CJ (2016)
Scalability and process transfer of mesenchymal stromal cell production from monolayer to microcarrier culture using human platelet lysate,
Cytotherapy, ISSN: 1477-2566. DOI:
10.1016/j.jcyt.2016.01.007.
Morris, TJ, Picken, A, Sharp, DMC, Slater, NKH, Hewitt, CJ,
Coopman, K (2016)
The effect of Me2SO overexposure during cryopreservation on HOS TE85 and hMSC viability, growth and quality,
Cryobiology, ISSN: 1090-2392. DOI:
10.1016/j.cryobiol.2016.09.004.
Murasiewicz, H, Nienow, AW, Hanga, MP,
Coopman, K, Hewitt, CJ, Pacek, AW (2016)
Engineering considerations on the use of liquid/liquid two phase systems as a cell culture platform,
Journal of chemical technology and biotechnology, ISSN: 0268-2575. DOI:
10.1002/jctb.5166.
Moutsatsou, P,
Coopman, K, Smith, M, Georgiadou, S (2015)
Conductive PANI fibres and determining factors for the electrospinning window,
Polymer, 77, pp.143-151, DOI:
10.1016/j.polymer.2015.08.039.
Heathman, T, Chan, A, Rafiq, Q,
Coopman, K, Nienow, A, Hewitt, CJ, Kara, B (2015)
Characterisation of human mesenchymal stem cells from multiple donors and the implications for large scale bioprocess development,
Biochemical Engineering Journal, 108, pp.14-23, ISSN: 1873-295X. DOI:
10.1016/j.bej.2015.06.018.
Heathman, T, Glyn, VAM, Picken, A, Rafiq, QA,
Coopman, K, Nienow, AW, Kara, B, Hewitt, CJ (2015)
Expansion, harvest and cryopreservation of human mesenchymal stem cells in a serum-free microcarrier process,
Biotechnology and Bioengineering, 112(8), pp.1696-1707, ISSN: 0006-3592. DOI:
10.1002/bit.25582.
Heathman, TRJ, Nienow, AW, McCall, M,
Coopman, K, Kara, B, Hewitt, CJ (2015)
The translation of cell-based therapies: clinical landscape and manufacturing challenges,
REGENERATIVE MEDICINE, 10(1), pp.49-64, ISSN: 1746-0751. DOI:
10.2217/RME.14.73.
Heathman, T, Stolzing, A, Fabian, C, Rafiq, QA, Nienow, AW,
Coopman, K, Kara, B, Hewitt, CJ (2015)
Serum-free process development: improving the yield and consistency of human mesenchymal stem cell production,
Cytotherapy, ISSN: 1477-2566. DOI:
10.1016/j.jcyt.2015.08.002.
Nienow, AW, Rafiq, QA,
Coopman, K, Hewitt, CJ (2014)
A potentially scalable method for the harvesting of hMSCs from microcarriers,
Biochemical Engineering Journal, 85, pp.79-88, ISSN: 1369-703X. DOI:
10.1016/j.bej.2014.02.005.
Robinson, NJ, Picken, A,
Coopman, K (2014)
Low temperature cell pausing: An alternative short-term preservation method for use in cell therapies including stem cell applications,
Biotechnology Letters, 36(2), pp.201-209, ISSN: 0141-5492. DOI:
10.1007/s10529-013-1349-5.
Sharp, DMC, Picken, A, Morris, TJ, Hewitt, CJ,
Coopman, K, Slater, NKH (2013)
Amphipathic polymer-mediated uptake of trehalose for dimethyl sulfoxide-free human cell cryopreservation,
Cryobiology, 67(3), pp.305-311, ISSN: 0011-2240. DOI:
10.1016/j.cryobiol.2013.09.002.
Ginai, M, Elsby, R, Hewitt, CJ, Surry, D, Fenner, K,
Coopman, K (2013)
The use of bioreactors as in vitro models in pharmaceutical research,
Drug Discovery Today, 18(19-20), pp.922-935, ISSN: 1359-6446. DOI:
10.1016/j.drudis.2013.05.016.
Rafiq, QA, Brosnan, KM,
Coopman, K, Nienow, AW, Hewitt, CJ (2013)
Culture of human mesenchymal stem cells on microcarriers in a 5 l stirred-tank bioreactor,
Biotechnology Letters, 35(8), pp.1233-1245, ISSN: 0141-5492. DOI:
10.1007/s10529-013-1211-9.
Nienow, AW, Rielly, CD, Brosnan, K, Bargh, N, Lee, K,
Coopman, K, Hewitt, CJ (2013)
The physical characterisation of a microscale parallel bioreactor platform with an industrial CHO cell line expressing an IgG4,
Biochemical Engineering Journal, 76, pp.25-36, ISSN: 1369-703X. DOI:
10.1016/j.bej.2013.04.011.
Rafiq, QA,
Coopman, K, Nienow, AW, Hewitt, CJ (2013)
A quantitative approach for understanding small-scale human mesenchymal stem cell culture - implications for large-scale bioprocess development,
Biotechnology Journal, 8(4), pp.459-471, ISSN: 1860-6768. DOI:
10.1002/biot.201200197.
Brosnan, K, Want, A,
Coopman, K, Hewitt, CJ (2013)
Multiparameter flow cytometry for the characterization of human embryonic stem cells,
Biotechnology Letters, 35(1), pp.55-65, ISSN: 0141-5492. DOI:
10.1007/s10529-012-1052-y.
Rafiq, QA,
Coopman, K, Hewitt, CJ (2013)
Scale-up of human mesenchymal stem cell culture: current technologies and future challenges,
Current Opinion in Chemical Engineering, ISSN: 2211-3398. DOI:
10.1016/j.coche.2013.01.005.
Chan, AKC, Heathman, TRJ,
Coopman, K, Hewitt, CJ (2013)
Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells,
Biotechnology Letters, pp.1-11, ISSN: 0141-5492. DOI:
10.1007/s10529-013-1422-0.
Ginai, M, Hewitt, CJ,
Coopman, K, Elsby, R, Surry, D, Fenner, K (2013)
The use of bioreactors as in vitro models in pharmaceutical research,
Drug Discovery Today, ISSN: 1359-6446. DOI:
10.1016/j.drudis.2013.05.016.
Sharp, DMC, Slater, NKH, Picken, A, Morris, TJ, Hewitt, CJ,
Coopman, K (2013)
Amphipathic polymer-mediated uptake of trehalose for dimethyl sulfoxide-free human cell cryopreservation,
Cryobiology, ISSN: 0011-2240. DOI:
10.1016/j.cryobiol.2013.09.002.
Want, AJ, Nienow, AW, Hewitt, CJ,
Coopman, K (2012)
Large-scale expansion and exploitation of pluripotent stem cells for regenerative medicine purposes: Beyond the T flask,
Regenerative Medicine, 7(1), pp.71-84, ISSN: 1746-0751. DOI:
10.2217/rme.11.101.
Brosnan, K, Want, A,
Coopman, K, Hewitt, CJ (2012)
Multiparameter flow cytometry for the characterization of human embryonic stem cells,
Biotechnology Letters, pp.1-11, ISSN: 0141-5492.
Coopman, K (2011)
Large-scale compatible methods for the preservation of human embryonic stem cells: Current perspectives,
Biotechnology Progress, 27(6), pp.1511-1521, ISSN: 8756-7938. DOI:
10.1002/btpr.680.
Coopman, K, Wallis, R, Robb, G, Brown, AJH, Wilkinson, GF, Timms, D, Willars, GB (2011)
Residues within the Transmembrane Domain of the Glucagon-Like Peptide-1 Receptor Involved in Ligand Binding and Receptor Activation: Modelling the Ligand-Bound Receptor,
MOLECULAR ENDOCRINOLOGY, 25(10), pp.1804-1818, ISSN: 0888-8809. DOI:
10.1210/me.2011-1160.
Coopman, K (2011) Large-scale compatible methods for the preservation of human embryonic stem cells: Current perspectives,
Biotechnology Progress, ISSN: 8756-7938.
Chippendale, TWE,
Coopman, K, Rafiq, Q, Hewitt, CJ, El Haj, AJ (2011) Isolation of Mesenchymal Stem Cells from Bone Marrow Aspirate,
Comprehensive Biotechnology, Second Edition, Volume 5, pp.115-123.
Coopman, K, Huang, Y, Johnston, N, Bradley, SJ, Wilkinson, GF, Willars, GB (2010)
Comparative Effects of the Endogenous Agonist Glucagon-Like Peptide-1 (GLP-1)-(7-36) Amide and the Small-Molecule Ago-Allosteric Agent "Compound 2" at the GLP-1 Receptor,
The Journal of Pharmocology and Experimental Therapeutics, 334, p.795, DOI:
10.1124/jpet.110.166009.
Coopman, K, Smith, LD, Wright, KL, Ward, SG (2007)
Temporal variation in CB2R levels following T lymphocyte activation: Evidence that cannabinoids modulate CXCL12-induced chemotaxis,
International Immunopharmacology, 7, pp.360-371, DOI:
10.1016/j.intimp.2006.11.2008.
Wright, KL, Weaver, SA, Patel, K,
Coopman, K, Feeney, M, Kolios, G, Robertson, DAF, Ward, SG (2004)
Differential regulation of prostaglandin E biosynthesis by interferon-gamma in colonic epithelial cells,
Bitish Journal of Pharmacology, 141, pp.1091-1097, DOI:
10.1038/sj.bjp.0705719.
Conferences
Grant, R,
Coopman, K, Gomes, S, Campbell, J, Braybrook, J, Petzing, J (2022)
Applying uncertainty analysis to assess the variation of operator performance when manually gating flow cytometry data [Poster presentation]. In
Midlands Innovation Flow Cytometry Meeting 2022, Loughborough, UK.
Grant, R,
Coopman, K, Gomes, S, Campbell, J, Braybrook, J, Petzing, J (2022)
Applying uncertainty analysis to assess the variation of operator performance when manually gating flow cytometry data [Abstract]. In
Midlands Innovation Flow Cytometry Meeting 2022, Loughborough, UK.
Grant, R,
Coopman, K, Medcalf, N, Gomes, S, Campbell, JJ, Braybrook, J, Petzing, J (2020) Applying uncertainty analysis to assess the variation of operator performance when manually gating Flow Cytometry data. In
CYTOTHERAPY,.
Grant, B,
Coopman, K, Medcalf, N, Silva-Gomes, S, Campbell, J, Kara, B, Braybrook, J, Petzing, J (2019)
Operator measurement uncertainty contributions within post-analytical flow cytometry data [poster]. In
4th PDA Europe Annual Meeting - Global Healthcare of the Present and the Future, Amsterdam, The Netherlands.
Grant, B,
Coopman, K, Mayer, S, Kara, B, Campbell, J, Braybrook, J, Petzing, J (2018)
Assessment of operator variation in flow cytometry measurements using gauge repeatability & reproducibility techniques. In
CYTOTHERAPY,, DOI:
10.1016/j.jcyt.2018.02.216.
Hanga, MP, Nienow, AW,
Coopman, K, Hewitt, CJ (2017)
A liquid/liquid two phase system as an economic alternative for the large-scale expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs). In
Journal of Biotechnology,, DOI:
10.1016/j.jbiotec.2017.06.694.
Nienow, AW, Rafiq, QA, Heathman, TRJ,
Coopman, K, Hewitt, CJ (2016)
Mixing theory for culture and harvest in bioreactors of human mesenchymal stem cells on microcarriers. In
, Theoretical Foundations of Chemical Engineering, pp.895-900, DOI:
10.1134/S0040579516060117.
Coopman, K (2014) Evaluating the effects of standard cryopreservation protocols on human mesenchymal stem cell survival and quality. In
, 4th International Conference on Stem Cell Engineering 2014, pp.261-269, ISBN: 9781510817623.
Nienow, AW, Rafiq, QA,
Coopman, K, Hewitt, CJ (2014) Mixing issues in stem cell culture. In
, 21st International Congress of Chemical and Process Engineering, CHISA 2014 and 17th Conference on Process Integration, Modelling and Optimisation for Energy Saving and Pollution Reduction, PRES 2014, p.822, ISBN: 9781510828131.
